Activation of adrenergic receptors by resveratrol elicits pigment aggregation in isolated melanophores of adult toad, Bufo melanostictus leading to skin lightening
نویسنده
چکیده
The present study was carried out to determine the effects of the seed skin extract of A. hypogaea along with active ingredient pure Resveratrol on the isolated dorsal skin melanophores of the toad Bufo melanostictus.The skin melanophores of the B. melanostictus, were assayed using the mean melanophore size index and their responses were recorded in the presence of various concentrations of the plant extract and its active ingredient along with speci! c antagonists and potentiator. It was found that the A. hypogaea extracts induced a signi! cant melanin aggregating effect making all the melanophores retard their processes gradually, making the dorsal skin appear lighter. The melanin aggregation effects on the isolated melanophores of the toad, B. melanostictus by seed skin extract of A. hypogaea were completely blocked by Propranolol and partially blocked by prazosin. These melanin granule aggregation and potentiating effects of reserpine to seed skin extract of A. hypogaea in the melanophores of B. melanostictus were found to be effectively blocked by adrenergic receptor blockers propranolol ( blocker -complete blockade) as well as prazosin ( blockerPartial blockade). In the present study the effect of seed skin extracts of A. hypogaea and its active ingredient Resveratrol induced a physiological as well as statistically signi! cant melanophore aggregation effect, leading to lightening of the isolated dorsal skin of toad, B. melanostictus
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